dot blot brdu rat monoclonal antibody  (Santa Cruz Biotechnology)

Journal: Brain, Behavior, & Immunity - Health

Article Title: AMPK activation improves depression-like symptoms in olfactory bulbectomized mice by regulating microglia M1/M2 polarization in the hippocampus

doi: 10.1016/j.bbih.2025.101008

Figure Lengend Snippet: Effect of AICAR on neuronal cell survival in the hippocampal DG of OBX mice. A: Time course of experimental protocol. B: Confocal images of brain slices stained with NeuN (green) and BrdU (red). The arrowheads indicate double-positive cells in the DG. C and D: Quantitative analysis of the number of BrdU + (C) and NeuN + /BrdU + (D) cells in the hippocampal DG. One-way ANOVA: F (2, 14) = 9.098, p = 0.0029, (C); F (2, 14) = 16.82, p = 0.0002, (D). Bars represent means ± SEM. ∗∗p < 0.01 vs. sham + vehicle group. ##p < 0.01 vs. OBX + vehicle group (n = 5–6 per group). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: After washing the sections for 5 min thrice, they were incubated with phosphate-buffered saline (PBS) containing 1 % normal goat serum or 1 % normal donkey serum and 0.3 % Triton X-100 (PBSGT or PBSDT, respectively) at room temperature (23 ± 1 °C) for 2 h. They were then incubated overnight at 4 °C with primary monoclonal antibodies against rat anti-BrdU (1:100; Harlan SeraLab, Loughborough, UK), mouse anti-neuronal nuclear antigen (NeuN; 1:500; Millipore, Burlington, USA), rabbit anti-CD86 (1:200; Thermo Fisher Scientific, Waltham, USA), goat anti-CD206 (1:500; R&D Systems, Minneapolis, USA), and rabbit anti-ionized calcium binding adaptor molecule 1 (Iba1; 1:200, Wako Pure Chemical Industries Osaka, Japan).

Techniques: Staining

Journal: Brain, Behavior, & Immunity - Health

Article Title: AMPK activation improves depression-like symptoms in olfactory bulbectomized mice by regulating microglia M1/M2 polarization in the hippocampus

doi: 10.1016/j.bbih.2025.101008

Figure Lengend Snippet: Effect of AICAR on hippocampal polarization of microglia in OBX mice. A and B: Confocal images of brain slices stained with DAPI (blue), Iba1 (green), CD86 (red) (A), or CD260 (red) (B). The boxed area is shown at higher magnification. C–E: Quantitative analysis of the number of Iba1 + /DAPI + cells (C) and the percentage of Iba1 + /CD86 + (D) and Iba1 + /CD206 + (E) cells in the hippocampus. F and G: Relationship between number of NeuN + /BrdU + cells in the hippocampus of DG and the percentage of Iba1 + /CD86 + cells (F) and Iba1 + /CD206 + cells (G). One-way ANOVA: F (2, 13) = 8.617, p = 0.0041, (C); F (2, 13) = 19.3, p = 0.0001, (D); F (2, 13) = 61.39, p < 0.0001, (E). Bars represent means ± SEM. ∗p < 0.05, ∗∗p < 0.01 vs. sham + vehicle group. ##p < 0.01 vs. OBX + vehicle group (n = 5–6 per group). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: After washing the sections for 5 min thrice, they were incubated with phosphate-buffered saline (PBS) containing 1 % normal goat serum or 1 % normal donkey serum and 0.3 % Triton X-100 (PBSGT or PBSDT, respectively) at room temperature (23 ± 1 °C) for 2 h. They were then incubated overnight at 4 °C with primary monoclonal antibodies against rat anti-BrdU (1:100; Harlan SeraLab, Loughborough, UK), mouse anti-neuronal nuclear antigen (NeuN; 1:500; Millipore, Burlington, USA), rabbit anti-CD86 (1:200; Thermo Fisher Scientific, Waltham, USA), goat anti-CD206 (1:500; R&D Systems, Minneapolis, USA), and rabbit anti-ionized calcium binding adaptor molecule 1 (Iba1; 1:200, Wako Pure Chemical Industries Osaka, Japan).

Techniques: Staining